The Novel Object Test measures animals’ behavioural reactions to an unfamiliar object. It is designed to assess curiosity, exploration, and fear-related responses toward novel stimuli. This test can be performed in different environments and is designed to test a single animal or multiple animals in one testing session. It is adaptable to all age classes. The test procedure described in this SOP is performed in a tie-stall environment, using either one of two different object presentation methods on either a single animal or multiple animals simultaneously.